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KMID : 0861020130280020039
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Korea Journal of Herbology
2013 Volume.28 No. 2 p.39 ~ p.44
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Comparison among GGEx16, GGEx18 and gambitongseong-capsule for anti-obesity gene activity
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오재호:Oh Jae-Ho
안예지:Ahn Ye-Ji/이혜림:Lee Hye-Rim/신순식:Shin Soon-Shik/임혜숙:Lim Hye-Sook/이형희:/윤미정:Yoon Mi-Jung
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Abstract
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Objectives : Gambigyeongsinhwan 16 (GGEx16), gambigyeongsinhwan 18 (GGEx18) and gambitongseong capsule
are shown to be involved in the regulation of obesity. Therefore, the aim of this study was to compare the
reporter activity of anti-obesity genes such as peroxisome proliferator-activated receptor α (PPARα) and PPARδ
by GGEx16, GGEx18 and gambitongseong capsule.
Methods : After NMu2Li liver cells, C2C12 skeletal muscle cells and 3T3-L1 preadipocytes were treated with
GGEx16 (1 μg/ml), GGEx18 (1 μg/ml) and different concentrations of gambitongseong capsule, the transactivation
of PPARα and PPARδ was measured by a luciferase reporter gene assay.
Results : PPARα reporter gene activity in NMu2Li liver cells and 3T3-L1 preadipocytes was significantly
increased by GGEx16, GGEx18 and gambitongseong capsule compared with control, whereas PPARα reporter gene
activity in C2C12 skeletal muscle cells was significantly increased by GGEx18 only compared with control.
Similarly, PPARδ reporter gene activity in 3T3-L1 preadipocytes was also significantly increased by GGEx18
compared with control. PPARδ reporter gene activity in C2C12 skeletal muscle cells was significantly increased by
GGEx16 and GGEx18 compared with control although PPARδ reporter gene activity in NMu2Li liver cells was not
changed by these three formulas.
Conclusions : These results suggest that all three formulas have the ability to stimulate PPARα and PPARδ
transactivation in animal cell lines with high metabolic rates. In particular, this effects were most prominent in
GGEx18-treated cells. In addition, it is likely that GGEx18 may be used as an effective anti-obesity composition.
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KEYWORD
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GGEx18, gambitongseong-capsule, PPARα, PPARδ, obesity
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